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Home > Literature List > Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice

Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice

Journal name:PLOS
Literature No.:
Literature Url: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167687
Date publication:December 9, 2016
Forsythia suspensa (Fsuspensa) is a traditional medicine for treatment of inflammation. In this study, we evaluated the therapeutic effects of an ethanol extract from Fsuspensa fruits on atopic dermatitis both in vivo and in vitro. We investigated the inhibitory effects of Fsuspensa extract on the development of atopic dermatitis-like skin lesions in an NC/Nga mouse model exposed to Dermatophagoides farinae crude extract. Topical application of Fsuspensa extract to the mice attenuated the atopic dermatitis symptoms, including increased dermatitis severity score, ear thickness, infiltration of inflammatory cells in the skin lesions, serum levels of IgE, TNF-α, and histamine, and expression of chemokines, cytokines, and adhesion molecules in ear tissue. In addition, Fsuspensa extract inhibited the production of chemokines in TNF-α/IFN-γ-activated human keratinocytes. High-performance liquid chromatography analysis of FSE revealed the presence of four chemical constituents (forsythiaside, phillyrin, pinoresinol, and phylligenin). These compounds inhibited the production of chemokines in TNF-α/IFN-γ-activated human keratinocytes. These results suggest that the Fsuspensa might be a useful candidate for treating allergic skin inflammatory disorders.


... The HaCaT cells were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C. Forsythiasidephillyrinpinoresinol, and phylligenin (Purity: 98%) were purchased from Chengdu Biopurify Phytochemicals (Chengdu, China). ...