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Home > Literature List > Comprehensive characterization of the in vitro and in vivo metabolites of geniposide in rats using ultra-high-performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometer

Comprehensive characterization of the in vitro and in vivo metabolites of geniposide in rats using ultra-high-performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometer

Journal name:Xenobiotica
Literature No.:
Literature Url: http://www.tandfonline.com/doi/abs/10.3109/00498254.2015.1079746#.Vf_TivnvPIU
Date publication:02 Sep 2015


?Yun Li, Wei Caiab, Qian Cai, Yanyun Che,Baosheng Zhao & Jiayu Zhang

Abstract

  1. ?Geniposide (genipin 1-O-glucose), one of the major bioactive constituents isolated from Fructus Gardeniae, possesses many biological activities. In this study, an efficient strategy was developed using ultra-high-performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometer (UPLC–LTQ–Orbitrap) to profile the in vitro and in vivo metabolic patterns of geniposide in rat liver microsomes (RLMs), plasma, urine, and various tissues. And post-acquisition data-mining methods including extracted ion chromatogram (EIC), multiple mass defect filters (MMDF), fragment ion searching (FISh), and isotope pattern filtering (IPF) were adopted to characterize the known and unknown metabolites.

   geniposide was purchased from Chengdu Biopurify    Phytochemicals Ltd. (Chengdu, China).

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