Abstract
Although mechanical ventilation (MV) is indispensable to life-support therapy in critically ill patients, it may promote or aggravate lung injury known as ventilator-induced lung injury (VILI). 6-Gingerol is the principal ingredient of ginger with potential anti-inflammatory and antioxidant properties in various diseases. Nevertheless, the role and mechanism of 6-gingerol in the process of VILI has not been explicitly investigated. In the study, we found that pre-treatment with 6-gingerol significantly improved the histological changes and pulmonary oedema, inhibited neutrophil accumulation and the release of early pro-inflammatory cytokines and MPO, and reduced oxidative stress reactions after high MV. Moreover, 6-gingerol treatment also increased PPARγ expression and decreased NF-κB activation in rats subjected to high MV. Furthermore, GW9662, a specific PPARγ inhibitor, was demonstrated to activatethe NF-κB pathway and cancele the protective role of 6-gingerol in VILI. This indicates that 6-gingerol exerted anti-inflammatory and antioxidative stress effects in VILI by activating PPARγ and inhibiting the NF-κB signalling pathway.
… 2. Materials and methods. 2.1. Materials and Reagents. Adult male Wistar
rats (200–250 g) were obtained from Yang Zhou University.
6-Gingerol was
bought from
Biopurify Phytochemicals Ltd, (Chengdu, China). GW9662 was …
