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Single-laboratory validation for the determination of caffeic acid and seven caffeoylquinic acids in sweet potato leaves

Journal name：BIOSCI BIOTECH BIOCH(BBB)
Literature No.：
Literature Url： http://www.tandfonline.com/doi/abs/10.1080/09168451.2014.942253#.VgJYlt_vPIU
Date publication：01 Aug 2014

Kazunori Sasaki, Tomoyuki Oki, Toru Kobayashi,Yumi Kai & Shigenori Okuno

Abstract

A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of caffeic acid (CA) and seven species of caffeoylquinic acids (CQAs) in lyophilized sweet potato leaves. The procedure for extraction of the analytes from the matrix and the HPLC conditions for the efficient separation of CA and CQAs were optimized. In the proposed method, a relative response factor to one of the CQAs (5-CQA) was used to quantify the others. The method performed well in terms of precision when carried out on five different days and demonstrated Horwitz ratio (HorRat) scores ranging from 0.5 to 1.0 for all analytes, which were well within the limits of performance acceptability. Accuracy testing at three levels showed an overall recovery of 94% when duplicated on five different days. Moreover, a stability study revealed that all analytes in both standard solution and

The other six CQAs were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China).