A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs

A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs

Honorine Willeman, Philippe Hance, Anne Fertin, Najia Voedts, Nathalie Duhal, Jean-Francois Goossens, and Jean-Louis Hilbert

Abstract

A method for the simultaneous determination of free chlorogenic acids (CGA) and sesquiterpene lactones (STL) in chicory root and its dried (flour) and roasted (grain) forms is described. The method uses one extraction and one analysis for all chicory root products. Various solvents with low to high polarity, such as methanol, chloroform, or n-hexane, were tested alone, in combination in different proportions or with acidified or neutral aqueous solvent. The water/chloroform/methanol (30/30/40, v/v/v) mixture generated the best extraction yield, 21% higher than alcohol mixtures. The profiling of CGA and STL content was performed through a conventional

The standards used for the HPLC analysis were 5-mono-O-caffeoylquinic acid (5-CQA) and 3,5-di-O-caffeoylquinic acid(3,5-diCQA) and were provided by Biopurify (Chengdu, China)