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Home > Literature List > Simultaneous quantification of vitamin E, γ-oryzanols and xanthophylls from rice bran essences extracted by supercritical CO2

Simultaneous quantification of vitamin E, γ-oryzanols and xanthophylls from rice bran essences extracted by supercritical CO2

Journal name:Food Chemistry
Literature No.:
Literature Url: http://www.sciencedirect.com/science/article/pii/S030881461630677X
Date publication:15 November 2016
Since the nutrition value of rice is diminished during rice processing, technology that can preserve and sustain functional compounds is necessary. In this study, supercritical carbon dioxide (SC-CO2) extraction was optimized for operational conditions (time, temperature, pressure and modifier) to extract vitamin E, γ-oryzanols and xanthophylls from rice bran. The simultaneous quantification of the compounds was developed using high-performance liquid chromatography with diode array and fluorescence detectors. Central composite design and respond surface methodology were applied to achieve optimum extraction conditions. The optimized conditions were 60 min, 43 °C, 5420 psi with 10% ethanol as a modifier. Pigmented rice bran extracts contained greater amounts of functional phytochemicals than non-pigmented rice bran extracts (0.68, 1410, and non-detectable μg/g compared with 16.65, 2480, and 0.10 μg/g of vitamin E, γ-oryzanols and xanthophylls in pigmented and non-pigmented ones, respectively). SC-CO2 extraction with modifier would be promising for preparation of phytochemical essences for therapeutic purpose.

... Ltd. (Japan). Standard γ-oryzanols (98%, Ichimaru Pharcos, Japan) were kindly provided by Prof.Dr. Malyn Chulasiri, and standard xanthophyll (
lutein) was obtained from Chengdu Biopurify Phytochemicals (China). 2.4. Experimental design for extraction of rice bran samples. ...
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