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Home > Literature List > Pachymic acid promotes induction of autophagy related to IGF-1 signaling pathway in WI-38 cells

Pachymic acid promotes induction of autophagy related to IGF-1 signaling pathway in WI-38 cells

Journal name:Phytomedicine
Literature No.:
Literature Url: http://www.sciencedirect.com/science/article/pii/S094471131730137X
Date publication:29 September 2017
Background

: The insulin-like growth factor 1 (IGF-1) signaling pathway has spotlighted as a mechanism to elucidate aging associated with autophagy in recent years. Therefore, we have tried to screen an effective compound capable of inducing autophagy to delay aging process.

Purpose

: The aim of this study is to investigate whether pachymic acid, a main compound inPoria cocos, induces autophagy in the aged cells.

Methods

: The aging of young cells was induced by treatment with IGF-1 at 50 ng/mL three times every two days. The effect of pachymic acid on cell viability was evaluated in human lung fibroblasts, WI-38 cells, using MTT assay. The induction of autophagy was detected using autophagy detection kit. The expression of proteins related to autophagy and IGF-1 signaling pathway was examined by western blot analysis and immunofluorescence assay.

Results

: In this study, pachymic acid showed cytotoxic effect in a dose dependent manner and remarkably induced autophagy at the same time. Moreover, pachymic acid increased the expression of proteins related to autophagy such as LC3-II and Beclin1 and decreased the levels of mTor phosphorylation and p70S6K in the aged cells. In particular, pachymic acid increased the expression of p-PI3K, p-FoxO and Catalase. In addition, pachymic acid remarkably increased the expression of IGFBP-3.

Conclusion

: Above results suggest that pachymic acid could induce autophagy related to IGF-1 signaling pathway in the aged cells.


... Co. (St. Louis, MO, USA). The purity of pachymic acid purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, Sichuan, CHN) is above 98% and was dissolved in dimethyl sulfoxide for further study. Cell culture. The ...
 
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