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Home > Literature List > Strategy for rapid screening of antioxidant and anti‐inflammatory active ingredients in Gynura procumbens (Lour.) Merr. based on UHPLC‐Q‐TOF‐MS/MS and characteristic ion filtration

Strategy for rapid screening of antioxidant and anti‐inflammatory active ingredients in Gynura procumbens (Lour.) Merr. based on UHPLC‐Q‐TOF‐MS/MS and characteristic ion filtration

Journal name:Biomedical Chromatography
Literature No.:
Literature Url: https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4635
Date publication:30 June 2019
Abstract

Gynura procumbens (Lour.) Merr. (G. procumbens) is traditionally used as a raw material for making dumplings or steamed stuffed bun, and its fresh leaves would be boiled with water for tea. Herein, we established an ultra‐high–performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry (UHPLC‐Q‐TOF‐MS/MS) combined with characteristic ion filtration (CIF) strategy to rapidly screen active ingredients with anti‐oxidant and anti‐inflammatory properties in G. procumbens. This strategy involved screening the active part of G. procumbens by anti‐oxidation and anti‐inflammatory activity assays; discovering the active compounds by speculating the active site's chemical composition by UHPLC‐Q‐TOF‐MS plus CIF; and verifying the active compounds’ activities. The ethyl acetate extract (EEAF) of G. procumbens was the major active site. Eighty‐one compounds were identified from the EEAF by the UHPLC‐Q‐TOF‐MS/MS plus CIF. Furthermore, polyphenols such as cynarine, isochlorogenic acids A and isochlorogenic acids C have excellent antioxidizing and anti‐inflammatory activities. This study provides a practical strategy for rapid in vitro screening of the antioxidizing and anti‐inflammatory activities of traditional vegetables and herbs and identification of active ingredients.


… Materials Capsaicin was obtained from Abcam (China). isochlorogenic acids A and isochlorogenic acids C were obtained from Chengdu Biopurify Phytochemicals Ltd (China). …