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Home > Literature List > A simple method to discriminate Guangchenpi and Chenpi by high-performance thin-layer chromatography and high-performance liquid chromatography based on analysis of dimethyl anthranilate

A simple method to discriminate Guangchenpi and Chenpi by high-performance thin-layer chromatography and high-performance liquid chromatography based on analysis of dimethyl anthranilate

Journal name:Journal of Chromatography B
Literature No.:
Literature Url: https://www.sciencedirect.com/science/article/pii/S157002321930827X
Date publication:15 September 2019
Abstract

Citri Reticulatae Pericarpium (CRP), the dried pericarp of Citrus reticulataBlanco, can be divided into “Guangchenpi” (GCP, the dried pericarps derived from Citrus reticulata ‘Chachi’) and “Chenpi” (CP, the dried pericarps derived from other cultivars of Citrus reticulata Blanco). To discriminate between GCP and CP, a simple and reliable high-performance thin-layer chromatography (HPTLC) method was firstly developed to analyze the volatile compound dimethyl anthranilate, and a high-performance liquid chromatography (HPLC) method was established to simultaneously quantify dimethyl anthranilate and three predominant flavonoids (hesperidin, nobiletin and tangeretin) in CRP samples. Both the HPTLC analysis and HPLC-orthogonal partial least squares discrimination analysis (OPLS-DA) indicated that GCP can be effectively distinguished from CP based on analysis of dimethyl anthranilate. Our results indicated that dimethyl anthranilate can be used as a marker compound for discrimination of GCP and CP. This work provided a convenient approach which might be applied for quality evaluation of CRP.

… b ND means not detected. 2.2. Chemicals and reagents. Reference standards of
hesperidin (C1), nobiletin (C2) and tangeretin (C3) were purchased from Chengdu
Biopurify Phytochemicals Co., Ltd. (Chengdu, China). Dimethyl … 
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