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Home > Literature List > Esculetin inhibits proliferation, migration, and invasion of clear cell renal cell carcinoma cells

Esculetin inhibits proliferation, migration, and invasion of clear cell renal cell carcinoma cells

Journal name:Biomedicine & Pharmacotherapy
Literature No.:
Literature Url: https://www.sciencedirect.com/science/article/pii/S0753332220302225
Date publication:May 2020
Abstract

Background

Esculetin, the main active ingredient in the Chinese herbal medicineCortex Fraxini, has been shown to possess antitumor activity. However, the effect of esculetin on clear cell renal cell carcinoma (ccRCC) has not been investigated.

Methods

MTS assays and colony formation assays were used to study the cytotoxicity of esculetin. The effects of esculetin on cell cycle and apoptosis were analyzed by flow cytometry. Western blot was used to detect cell cycle-, apoptosis-, and EMT-related proteins. Wound-healing assays and transwell assays were performed to study the effect of esculetin on cell migration and invasion in the ccRCC cell lines 786-O and SN12-PM6.

Results

Esculetin exerted cytotoxic activities in 786-O and SN12-PM6 cells in a dose- and time-dependent manner. The compound arrested the cell cycle in G0/G1 and G2/M phase with down-regulation of Cyclin D1, CDK4, CDK6, and c-Myc expression. Esculetin also induced apoptosis and the expression of cleaved caspase 3 increased. Additionally, esculetin significantly inhibited 786-O and SN12-PM6 cell migration and invasion, the expression of E-Cadherin increased, and the expression of N-cadherin and vimentin decreased.

Conclusion

Taken together, these results suggest that esculetin inhibits proliferation, migration, and invasion of ccRCC and is a potential novel therapeutic agent for the treatment of ccRCC.

… China) under 5% CO 2 at 37℃. Esculetin was obtained from Chengdu Biopurify
Phytochemical Ltd. (Chengdu, China) and dissolved in dimethyl sulfoxide (DMSO,
company name). 2.2. MTS assay. Cells at a density of 5 × 10 … 
 
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