Abstract
Pyrrolizidine alkaloids (PAs) are naturally occurring plant toxins associated with severe liver damage if excessive ingestion. Herein, a novel analytical strategy on utilizing direct analysis in real-time mass spectrometry (DART-MS) was developed, and applied in analysis of six representative PAs. The calibration curves in the range of 10–1000 ng·mL−1 were established, and relative standard deviations (RSDs) were less than 10%. The limits of detection (LODs) and limits of quantitation (LOQs) were 0.55–0.85 ng·mL−1 and 1.83–2.82 ng·mL−1, respectively. The feasibility of method was indicated by analysing real samples including Gynura japonica, drug tablets, granules, and fresh cow’s milk. Moreover, the results of DART-MS were in good agreement with those observed by high performance liquid chromatography mass spectrometry (HPLC-MS), but consumed less time without chromatographic separation. This research provides a facile fashion for safety assessment of herbal and food products containing PAs and presents promising applications in food, pharmaceutical and clinical analysis.
… purification unless otherwise specified.
Senecionine (SEN),
seneciphylline (SENP),
senkirkine (SENK) and
retrorsine (RETRO) were purchased from Chengdu
Biopurify Phytochemicals Ltd. (Chengdu, China). Adonifoline (ADO) and …
